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The culture medium was replaced with neuronal conditioned serum-free B-27/Neurobasal medium (Gibco, Life Technologies, cat.# 17504-044 for B27 and cat.# 21103-049 for Neurobasal) 2 h after plating

Some contraindications (reasons not to take B12 shots) include: Allergies to cyanocobalamin or methylcobalamin Kidney or liver disease Eye issues, especially if you have Lebers disease A current infection If youre taking medication related to bone marrow You should talk to your doctor about the above conditions before getting B12 injections