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Western blot The total protein extracts were prepared using RIPA buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM Na3VO4, 5 mM NaF, and 1% cocktail protein protease inhibitors (Sigma)

Conscious effort to maintain daily movement helps counter NEAT reduction

Weight loss rates do slow over time, but this reflects a natural plateau as you approach a lower body weight rather than the medication becoming less effective

stenosis increases with age Occupation: Jobs requiring heavy lifting, twisting, or prolonged sitting Sedentary lifestyle: Weak core muscles fail to support the spine Obesity: Excess weight increases spinal stress Diabetes: Increases risk of nerve damage Smoking: Reduces disc nutrition and accelerates degeneration Diagnostic Evaluation Physical examination includes neurological testing of strength, sensation, and reflexes in the affected leg

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Some of them are working additional jobs or delaying retirement so they can continue to pay for it. ___ AP Health Writer Matthew Perrone contributed to this report