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Cells were transduced with lentiviruses in the presence of 10g/mL polybrene (Sigma, 107689), selected and maintained in Complete media containing 2g/mL puromycin (Bioshop, PUR333) for 2-3 days
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This therapy works best for the patient who plays a long game and wants their internal machinery to perform as well as the rest of their plan
HCV NS5A abrogates p53 protein function by interfering with p53-DNA binding
However, it may have little effect once the damage becomes too advanced
J.BrockD
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