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Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

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While niacinamide works to affect pigmentation, L-cysteines approach involves supporting glutathione production, which has a skin-lightening efficacy in humans

Standard protocols were followed for calibration of the chambers

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As for post-care, youll want to avoid pressing and manipulating the treatment area for 36 hours, to allow filler to naturally settle into its new home