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For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

Compared to normal aging and D-GAL-induced premature aging models, research on endothelial cell senescence induced by hypoxia under pathological conditions remains scarce

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This avoids potential interactions between the copper peptide and retinoid in the same application

Bioconjugate Chem