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Additional specificity analysis was conducted to compare the outcomes of GLP-1 RA users with those of patients receiving other second-line antihyperglycemic treatments, including thiazolidinediones (TZDs), DPP-4is, or sulfonylureas (SUs)

Besides, cells were also collected for western blot analysis to determine the expression of endothelial markers [VE-cadherin, zonula occludens-1 (ZO-1)] and mesenchymal markers [alpha smooth muscle Actin (-SMA) and Vimentin]

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Dual GLP-1/GIP agonists, such as tirzepatide, primarily enhance glycemic control and insulin sensitivity through synergistic incretin and -cell effects, producing the most pronounced HbA1c reductions among all classes

This creates a beneficial cycle: lifestyle changes that promote GLP-1 release support weight management, which in turn may enhance GLP-1 function