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2.9 Assay of hepatic Nrf2 and NF-B gene expressions 2.9.1 RNA extraction The RNeasy QIAamp -Mini kit (Qiagen, Hilden, Germany) was used for the extraction of RNA from tissue samples after 30 mg of tissue was added to 600 L of RLT buffer containing 10 L of-mercaptoethanol per 1 mL
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TLR pathway activation leads to secretion of pro-inflammatory cytokines, like interleukin (IL)-1, IL-6, and tumor necrosis factor-, as well as type 1 interferon
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