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In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out

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Common Side Effects Gastrointestinal: Nausea, vomiting, abdominal cramps, and diarrhea the most common adverse effects, occurring primarily at doses exceeding 3 g/day [1][5][7]

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