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Required Equipment and Materials Plate reader (405 or 415 nm filter) 96-well microplate Incubator (37C) 20 l and 200 l pipettes, a multi channel pipette 5-Sulfosalicylic Acid (SSA) Solution Ethanol Assay Procedure Sulfasalazine Alters Intracellular Metabolites and Increases ROS in A549 Cells Induction of Ferroptosis by Erastin Erastin is a known inducer of ferroptosis
e , Gene expression of indicated genes involved in thermogenesis in BAT, inguinal WAT, and gWAT from Control and NDI1 mice after 12 weeks of HFD feeding (n = 8)
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MDHA is either directly reduced back to AsA by MDHAR or undergoes non-enzymatic disproportionation to AsA and dehydroascorbate (DHA)
