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Together, these therapeutic advances underscore the emergence of cardiac proteostasis as a druggable, systems-level target

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After blocking with 5% bovine serum albumin (BSA, Sigma-Aldrich) in TBST (10 mM Tris-HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 C