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Add 5ml B complex vial into infusion bag
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Calibration standards were prepared by spiking 50 L of human plasma with appropriate amount of thiols to obtain the following concentrations: 20, 40, 60, 100, 200, 300, 600 nmol/mL plasma for Cys, 2, 4, 6, 10, 20, 30, 60 nmol/mL for Hcy, GSH and 0.1, 0.5, 1.0, 2.0, 4.0, 5.0, 10.0 nmol/mL for N-Hcy-Lys
2.4.4.2 Liquid chromatography-mass spectrometry analysis conditions The analyzing instrument was a liquid-mass spectrometry system consisting of a Waters ACQUITY UPLC I-Class plus/Thermo QE ultra-high performance liquid tandem high-resolution mass spectrometer