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(2) To remove hydrophobic substances, wash the column with 3-4 bed volumes of 70% ethanol or 2 bed volumes of 1% Triton X-100 followed by 5 bed volumes of PBS (pH 7.4) washed

For a clinician-facing summary of timing and management considerations see this safety review

Bathel et al

If meals start feeling like a task you keep skipping, that is the cue to ease off a dose, not to admire how little you can get by on

Mice were housed under standard laboratory conditions with controlled temperature and humidity, a 12 h light/dark cycle, free access to food and water, and environmental enrichment