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After washing and resuspension in 24 M fluorescein solution in HBSS, TR-FRET was monitored before and after agonist addition in a Flexstation 3 plate reader at 37C using the following settings: ex = 335 nm, em = 520 and 620 nm, delay 400 s, integration time 1500 s
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Neither medication has yet demonstrated reduced cardiovascular events in dedicated outcome trials, though these studies are ongoing
Weeks 13 through 16: 1.7 mg semaglutide per week if tolerated
Researchers specifically investigating MC4R-mediated biology often use MT-2 as the non-selective melanocortin agonist probe, with multi-receptor activity accounted for in the experimental design
