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Stress Reporter Assay of AQ-QI and DEAQ-QI To identify the stress response pathways upon AQ-QI and DEAQ-QI exposure, oxidative stress response (SRXN1-GFP), ER stress response (CHOP-GFP), inflammation response (ICAM1-GFP), and DNA damage response (p21-GFP) pathways were measured with a high-content image-based BAC-GFP stress responses reporter assay ( HepG2-based reporter cells were generated by Bacterial Artificial Chromosome tagging as described previously ( 4 per well in 0.1 mL DMEM, treated subsequently as described above

This protective shell helps the glutathione bypass the digestive enzymes in the stomach, allowing it to be absorbed more efficiently in the small intestine

PMID: 21164074

197 Gut microbiome composition is usually altered in CKD patients

Analysis suggested an early stress induced changes in the expression of genes with functional redundancy and precise involvement of GSTs in the oxidative stress protection (Sappl et al., 2009)

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