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Indexed samples were pooled in an equimolar amount (4 nM), adjusted to a final concentration of 4 pM and sequenced on a MiSeq system (Illumina) in a paired-end reaction of 600 cycles using the MiSeq reagent kit v3 (Illumina)
Through receptor mediated signaling, Cagrilintide modulates pathways linked to appetite signaling circuits, gastrointestinal regulatory mechanisms, and broader metabolic feedback systems examined in controlled experimental models
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