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The antibody Fab7F38 appears to function by providing enhanced soluble surface area for crystal lattice packing, and indeed Fab7F38-bound GLP-1R can also assume an active conformation as shown by EM studies (see below), suggesting that Fab7F38 does not interfere with the conformational flexibility of the ECD during crystallization

doi: 10.1016/j.jmb.2006.11.076 113

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The fixed-ratio nature of the blend also means components cannot be titrated independently: any adjustment scales all four peptides together