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To estimate the correlation between changes in the expression of (h)GSH biosynthesis-related genes and actual thiol levels, a quantitative determination of low-molecular-weight thiols in 1-, 2-, and 3-week-old roots and nodules was undertaken (Figures 6A,B)
Free GSH concentration in the sample is calculated from the difference between two separate measurements the total GSH measurement and a measurement of the GSH generated from GSSG
Additionally, studies evaluating the role of ATs in human diseases rarely define which specific ROS are involved, their sources, how they reach their molecular targets, and which targets are affected, and why
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A magnesium deficiency can result in low glutathione levels and various symptoms (cramps, anxiety, joint pain, constipation, migraines, fatigue, depression, etc.)
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