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Supplied exclusively for laboratory research use.

Cells were washed with PBS, dissociated in 10 mM EDTA (2 ml per well) at 37 C for 5 min, and washed three times in cold PBS

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They supply the body with the necessary nutrients to repair and rebuild damaged tissues

Floating fraction was collected after 1 h, transferred into non-coated cell-culture flasks and incubated in suspension overnight

Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere