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Upon receptor engagement, BPC-157 initiates downstream phosphorylation cascades involving phospholipase C-gamma and protein kinase C activation, measurable through Western blot analysis and fluorescence-based kinase activity assays

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This far exceeds the affinity of many endogenous copper-chelating agents, and it matters because that tight coordination is what allows GHK-Cu to donate copper to cuproenzymes and initiate downstream gene expression cascades