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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

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doi:10.1111/resp.12927 Brown RE, Basheer R, McKenna JT, Strecker RE, McCarley RW