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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

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In terms of peripheral contributions, surgical patients often have increased exhausted T-cells and monocytes that secrete pro-inflammatory mediators affecting the brain

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