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These systems were able to replicate not only the cellular differentiation process observed in adherent (monolayer) cultures of stem cells, but also the spatial and morphogenic patterns involved in the three-dimensional (3D) development of certain neural structures (Lancaster and Knoblich (2014) took this approach to a higher level, generating the so-called cerebral organoids, which were described as an in vitro 3D-culture system capable of generating different brain regions, with a brain-like organization in human development (Lancaster et al., 2013)

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