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Obesity is the leading cause of non-alcoholic fatty liver disease (NAFLD) which, along with its downstream consequencessteatohepatitis, cirrhosis, and liver canceris the most common liver disease in the world (Araujo, Rosso, Bedogni, Tiribelli, & Bellentani, 2018)

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After 2 d of TGR dsRNA treatment, TGR activity (using DTNB as substrate) was reduced by 35% in parasites in aerobic culture (20% O 2 ) and after 3 d of TGR dsRNA treatment, TGR activity was reduced by 61% or 63.5% in parasites cultured anaerobically or aerobically, respectively

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