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The European Respiratory Journal Geiler J at al., (2010), N-acetyl-L-cysteine (NAC) inhibits virus replication and expression of pro-inflammatory molecules in A549 cells infected with highly pathogenic H5N1 influenza A virus, Biochemical Pharmacology McCarty at al.(2020), Nutraceuticals have potential for boosting the type 1 interferon response to RNA viruses including influenza and coronavirus, Progress in Cardiovascular Diseases Palacio at al., (2011) Anti-inflammatory properties of N-acetylcysteine on lipopolysaccharide-activated macrophages, Inflammation Research Sadowska et al.,(2006), Role of N-acetylcysteine in the management of COPD, International Journal of Chronic Obstructive Pulmonary Disease Schmitt et al.,(2015), Effects of N-acetylcysteine, oral glutathione (GSH) and a novel sublingual form of GSH on oxidative stress markers: A comparative crossover study, Redox Biology Zhang et al.,(2018), N-acetylcysteine improves oxidative stress and inflammatory response in patients with community acquired pneumonia, Medicine

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After washing the plates twice with wash buffer, Biotin-PCSK5 was added to the plates and incubated at 37 C for 2 h