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Immunoblotting Cells were washed with PBS and lysed with lysis buffer (150 mM NaCl, 20 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40) containing protease and phosphatase inhibitors

Sphingosine, considered the backbone of sphingolipids, is a key component of cell membranes, and involved in cell signaling, membrane structure, and are associated with cell-growth arrest and the induction of cell death 92,93

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doi:10.1017/S0029665121003700