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Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

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J Biol Chem 274(33):2293222940 Lu CW, Lin TY, Huang SK, Wang SJ (2016) Echinacoside inhibits glutamate release by suppressing voltage-dependent Ca2 + entry and protein kinase C in rat cerebrocortical nerve terminals

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It does not provide reconstitution, dosing, injection, administration, personal-use, veterinary-treatment, or clinical instructions