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Introducing outside chemicals or alternative peptide structures directly into the vial can alter the pH, disrupt the molecular stability, or cause the components to precipitate out of the liquid solution

The assay was designed to demonstrate the biochemical activity of c-met binding sites including Y198 and Y250 (in K1 and K2 domains of HGF, respectively) by significant changes in optical absorbance (expressed as relative unit) within a range from a baseline level (a negative control without HGF