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doi: 10.1093/hmg/ddt032 281 SchumannGLiuCOReillyPGaoHSongPXuBet al
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The exact timing depends on the patients needs, like how severe the deficiency is and how well they absorb the vitamin
Standard or sample was transferred in triplicate to a 96-well plate and GSH was then derivitized by the addition of 10 mM 2,3-naphthalenedicarboxyaldehyde and the fluorescence at Ex/Em wavelength of 472/528 nm was quantified using a fluorescent plate reader (Synergy 2, BioTek)
