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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min
High concentrations for IV use should be diluted in normal saline or another compatible IV fluid before administration
While not toxic, excessively high doses are usually unnecessary
Personalized dosing ensures maximum benefit and safety
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