Abbreviations ME: -mercaptoethanol BrdU: 5-bromo-2-deoxyuridine BSA: Bovine serum albumin DAB: 3,3-diaminobenzidine DMEM: Dulbeccos modified Eagles medium ECL: Enhanced chemiluminescence GSSSG: Glutathione trisulfide HGF: Hepatocyte growth factor HRP: Horseradish peroxidase HS: Horse serum mAb: Monoclonal antibody LASSS: Lipoic acid trisulfide SDS: Sodium dodecyl sulfate TTBS: Polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline Y: Tyrosine residue References Honda, S
Funding: This work was partially supported by the National Natural Science Foundation of China (82274379), Fundamental Research Funds for the Central Universities (2022-JYB-JBZR-009), and Beijing University of Chinese Medicine High-level Talent Start-up Research Project (2021-XJ-KYQD-004)
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CoQ10 acts as the primary electron carrier, accepting electrons from Complex I and Complex II and physically transporting them to Complex III