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Gather the injection pen, new needle, and alcohol swab

Since vitamin B12 is mostly found in animal products, those on a vegan or plant-based diet are also at a higher risk of being vitamin B12 deficient

Pre-filled pens/vials can also carry the risk of foreign body obstruction in the gastrointestinal tract and mechanical injury due to sharp components

Erickson, A.M., et al., Identification of a variant antioxidant response element in the promoter of the human glutamate-cysteine ligase modifier subunit gene

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Sakura Finetek Japan), snap-frozen in liquid nitrogen, and sectioned into 5-m slices using a cryostat (CM1950