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FIGURE 3 6

Glutathione levels decline with age, which is why supplementation is recommended

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Boc-His(Trt)-Aib-Glu(OtBu)-Gly-OH (fragment- 1) Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-OH (fragment-2) Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (fragment-3) Fmoc-Gln(Trt)-Ala-Ala-Lys(C18 diacid mono-t -butyl -y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-Ile-Ala-Trp(Boc)-OH (fragment-4) Fmoc-Leu-V al-Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu (fragment- 5) b) condensing Fmoc-Leu-V al-Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu (fragment-5) with Fmoc- Gln(Trt)-Ala-Ala-Lys(C18 diacid mono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-Ile-Ala-Trp(Boc)-OH (fragment-4) in presence of coupling agent and solvent in in-situ manner followed by deprotection in presence of base to obtain 15 amino acid peptide chain

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