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however, the mechanisms for this imbalance have been unclear (22)

The amount of protein used per sample was 12 g, and the PageRuler Prestained Protein Ladder 10180 kDa (Thermo Fisher Scientific Inc., Waltham, MA, United States) was used as molecular weight marker

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That said, severe pain has been reported by some people, especially when bigger needles or medication doses are used

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