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Mice subjected to MIA injection exhibited progressive motor dysfunction, characterized by shorter stay times on the rotarod compared to the sham group (Fig
Tesamorelin (a GHRH analog) opens one
21 This protective phenotype has been linked to estradiol-dependent enhancement of endogenous radical-trapping capacity, including hydroxylated estrogen metabolites and increased glutathione hydropersulfide buffering
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Positive clones were selected for by puromycin treatment, single-cell sorting, cell expansion, and confirmation by gene amplification and sequencing for correct gene cassette insertion, and immunoblotting for expression of the Cas9 protein after doxycycline treatment
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