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The shot itself takes seconds, plus a couple of minutes of intake

Yes, but balance the dosage to avoid neutralizing effects

In the second step, conjugating enzymes perform addition reactions (e.g., acetyl, methyl, glucuronic acid) on these newly modified, electrophilic substrates

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Methylation and methyl donor status NNMT consumes methyl donors as part of its enzymatic function

Nat Protoc 6(10):16121631