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Indeed, Leishmania -derived PUFA has been shown to directly influence the polarization of macrophages toward the M2 phenotype (119)

Briefly, during phase 1, cells were grown in Iscoves Modified Dulbecco medium (IMDM) with 15% fetal bovine serum (FBS), 15% human AB serum, 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2 IU/mL erythropoietin (Sigma-Aldrich, St