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Sections were then washed twice with PBS for 5 min each on a shaker, followed by incubation with a secondary antibody (donkey anti-rabbit, Invitrogen A32794, 1:500) for 30 min at room temperature
Core body temperature drop is a critical sleep onset trigger
As a synthetically produced peptide, ipamorelin is chemically similar to GHRP-1, the growth hormone-releasing peptide thats naturally produced in the body
doi:10.1016/j.phymed.2007.09.001
A tetrasubstituted 29-amino acid peptide analog of Growth Hormone Releasing Hormone (GHRH)
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