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To evaluate f-Cy5-GEP44 localization in Y1-R, Y2-R, and/or GLP-1R-expressing cells in the rat hindbrain, we performed RNAscope fluorescent in situ hybridization (FISH) as previously described 53 combined with immunohistochemistry (IHC) of coronal sections prepared from the rat brainstem

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Left: normal liver tissues

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