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After primary incubation, slides were washed in PBST (35 min), incubated with fluorophore-conjugated secondary antibodies for 1 h at RT, and washed again in PBST (35 min)
Severe calorie restriction in perimenopause can actually make things worse by: Further decreasing metabolic rate (adaptive thermogenesis) Increasing cortisol (chronic stress response) Promoting muscle loss over fat loss Disrupting already-irregular hormone patterns Ive seen women eating 1200 calories and doing intense cardio six days per week, completely exhausted and still gaining weight
Common side effects are gastrointestinal (nausea, diarrhoea, vomiting) and typically occur during initial treatment or dose increases
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A shorter half-life necessitates more frequent administration to maintain a steady, therapeutic level
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