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Probe 1426 in the presence of Ampligase (Lucigen) was used to selectively circularize and protect fragments containing the barcoded transposon sequence, while all other fragments were digested with a mix of T7 gene 6 exonuclease (ThermoFisher), Lambda exonuclease (NEB), and Exonuclease I (NEB)

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However, these technologies are largely unsuitable for use in a progressive GBM model as they primarily employ a single or few electrodes, rather than configurations that allow high-density mapping