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Cold injections can be uncomfortable

[9] Synthesis and function of sulfur compounds in plants [edit] Cysteine [edit] Sulfide is incorporated into cysteine, catalyzed by O-acetylserine (thiol)lyase, with O-acetylserine as substrate

doi: 10.1016/S0021-9258(18)77062-2

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Owing to its high concentration in the cell (~10 3 M) 1 , the GSH/GSSG pair acts as the main intracellular redox buffer, plays a key role in the detoxification of reactive oxygen species (ROS), and participates in redox signalling 2

The samples were then incubated in the dark at room temperature for 20 min