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Cryo-EM sample preparation and data acquisition Four microliters of the sample (protein concentration: 3 mg/mL, liposome concentration: 1.5 mg/mL) were loaded onto a Quantifoil Au grid (R2/2, 300 mesh), followed by plunge-freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) at 4 C and 100% humidity
The active molecule is identical
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Pre-incubation and dilution assay GSTO1 (2 g) was incubated with inhibitor at a concentration of 10 IC 50 in a 10 l reaction volume for 30 min at 37 C
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