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NAD+ levels naturally decrease by approximately 50% between ages 40 and 60, contributing to fatigue, cognitive fog, and reduced cellular repair capacity

The expression of target genes was analysed via quantitative real-time PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

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By supporting mitochondrial function and protecting against oxidative damage, glutathione injections may help improve cellular energy production and overall vitality

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Hensley 2009)