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Keep the vial upright to reduce contact between the rubber stopper and the solution

Quantification of mitochondrial DNA copies To measure the number of hepatic and renal mitochondrial DNA copies the total RNA was first extracted from hepatic and renal tissues using the Gene JET RNA Purification kit (Thermo-scientific, USA), converted the extracted RNA into complementary DNA (cDNA) based on the manufacturer instructions of Revert Aid First Strand cDNA Synthesis kit (Thermo-scientific, USA)

Researchers must recalculate the conversion whenever they change either the vial size or the reconstitution volume, since both variables independently affect the GHK-Cu dosing volume drawn into the syringe for each administration

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Secondary objectives were the evaluation of B12 and MMA concentrations in those two groups

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