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Metabolic assays For real-time measurement of the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), 2 10 5 in-vitro-polarized T H 1 cells were washed with Seahorse XF RPMI medium (pH 7.4) supplemented with 5 mM glucose (or without), 1 mM pyruvate, 2 mM glutamine, 100 g ml 1 linoleic or oleic acid and 5 mM NaCl or BHB, respectively

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