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The porcine pi-class enzyme pGTSP1-1 was the first GST to have its structure determined, and it is representative of other members of the cytosolic GST superfamily, which contain a thioredoxin-like N -terminal domain as well as a C -terminal domain consisting of alpha helices
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All proteins within an approximate radius of 10 nm 65 to either tagged NHR-49 fusion protein were biotinylated, affinity-purified, and subjected to LC-MS/MS 48
