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HEK-293T were from American Type Culture Collection (ATCC), and cultured in Dulbeccos modified Eagles medium (DMEM) (Cytiva, SH30003.02) supplemented with 10% fetal bovine serum (FBS, Cytiva)

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Semaglutide led to an average bodyweight reduction of 14.9% over 68 weeks

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Clinical studies have confirmed that hyperhomocysteinemia is not only an independent risk factor for the development of cardiovascular and cerebrovascular diseases, such as atherosclerosis, acute myocardial infarction, stroke, coronary artery lesions, and peripheral vascular disease, but also increases the risk of neurodegenerative disorders and tumors, and impairs the efficacy of antifolate drugs

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