Purification of the digested samples was carried out via an ion exchange chromatography (Mono Q 5/50 GL column GE) with the running buffer: 10 mM Tris-HCl pH 7.8 and a size exclusion chromatography on a Superdex 200 increase column (GE) using a running buffer: 200 mM NaCl, 50 mM Tris-HCl, pH 7.8
Vascular and endovascular surgery
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Before starting Melanotan, we should talk to a healthcare provider
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