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Malabsorption can cause severe B12 deficiency

doi: 10.1007/978-94-007-6889-5_13 21 LinYJiangMChenWZhaoTWeiY

Although glutamate is the major downstream product of glutamine, glutamate supplementation during glutamine deprivation cannot rescue the impaired cell growth or mitochondrial respiration 16,43,44,45 , indicating that mitochondrial GLS-catalyzed cleavage of the gamma-nitrogen of glutamine is essential for glutaminolysis

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Resolving the relative contributions of these mechanisms in vivo remains an important challenge for the field and will likely require quantitative imaging approaches capable of linking molecular events to tissue-scale dynamics