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Additionally, the cyclic trisulfide structure of LASSS, as opposed to the linear trisulfides such as GSSSG and cysteine trisulfide (Cys-SSS-Cys), could guarantee the unique reactivity of LASSS (see again Figure 1a for chemical structures)

For a broader overview of peptide injection techniques , the fundamentals apply across all subcutaneous peptides

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Firth, J

Dried peptides were resuspended in 0.1% TFA and Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) performed in single replicate injections as previously described 93 using an Ultimate 3000 nano-HPLC coupled to a Q-Exactive mass spectrometer (Thermo Scientific) via an EASY-Spray source

Standard cycles (8-12 weeks) allow deeper tissue effects to manifest